human htert immortalized foreskin fibroblast bj 5ta cells  (ATCC)

Journal: Molecular oncology

Article Title: Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence.

doi: 10.1002/1878-0261.70039

Figure Lengend Snippet: Fig. 5. Changes in ANT1, ANT2, and ANT3 protein and transcript levels in various types of cellular senescence. The ANT1, ANT2, and ANT3 (A) protein levels detected by lLC-PRM and (B) transcript levels normalized to b-actin transcript level detected by qPCR were determined in different senescent models and corresponding proliferating control. The mean of fold change between the treatment and proliferating control, calculated from three experimental replicates, is shown. (A, B) The data of IRIS RPE-1 cells (20 Gy; D14) from Fig. 4A,C are shown again for easier comparison of all senescence models. Error bars represent standard errors. (C) The correlation of log2 fold change of ANT mRNA and protein levels was calculated. Spearman’s correlation coefficient (Spearman) and P-value are shown. The tested models include contact inhibition (RPE-1 CI), IRIS (RPE-1 20 Gy), p16 and p21 CDK-inhibitor overexpression (RPE-1 p16 and RPE-1 p21) in RPE-1 cells, docetaxel-induced senescence in normal BJ fibroblasts (BJ DTX), and TMZ-induced senescence in glioblastoma cell lines (A-172 TMZ and U- 87 MG TMZ), calculated from three experimental replicates. Spearman’s rank correlation coefficient was used for statistics.

Article Snippet: Telomerase-immortalized human retinal pigment epithelial cell line hTERT RPE-1 (RPE-1), human foreskin fibroblasts (BJ), and human glioma A-172 and U87 MG cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA, cat. no. CLR-4000, CRL-2522, CRL-1620, HTB-14, respectively).

Techniques: Control, Comparison, Inhibition, Over Expression